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Rna interference thesis

This natural mechanism for sequence-specific gene silencing promises to revolutionize experimental biology and may have important practical applications in functional genomics, therapeutic intervention, agriculture and other areas. A possible mechanism underlying the regulation of endogenous genes by the RNAi machinery was suggested from studies of C. A simplified model for the RNAi pathway is based on two steps, each involving ribonuclease enzyme. Rather, gene silencing is a result of translational inhibition.
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RNA Interference (RNAi)

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People | Jockusch Lab

Purpose: Mutations of keratoepithelin KE and myocilin MYOC have been linked to certain types of inherited corneal stromal dystrophy and open-angle glaucoma, respectively. In this study, the feasibility of using small interfering RNAs siRNAs to suppress the expression of keratoepithelin and myocilin and their capabilities to reduce the related cytotoxic effects caused by mutant myocilins were investigated. A BiP promoter-driven luciferase reporter assay was used to evaluate the stress response of TM cells induced by misfolded mutant myocilins. The activation of BiP gene as a stress response induced by mutant myocilins in transformed TM cells was significantly reduced when mutant myocilin proteins were suppressed by myocilin-specific shRNAs. Conclusions: KE- or myocilin-specific shRNAs could effectively suppress the expression of recombinant KE or myocilin proteins and the related cytotoxicity of mutant myocilins.
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Jennifer Doudna

The development of a complex nervous system requires the actions of intricate genetic mechanisms that influence and maintain the differentiation of common nervous system progenitor cells into neurons and glial cells. In Drosophila melanogaster, the gene glial cells missing gcm encodes the transcription factor Gcm that, when active, causes early nervous system cells to preferentially differentiate into glial cells by activating the reversed polarity repo gene, as well as others. However, the Gcm protein also plays a role in the differentiation of both macrophages and tendon cells.
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I declare that this thesis was composed by me and all presented results are my own unless stated otherwise. All sources and literature that I have used are cited with reference to the corresponding source quoted in the text. It is possible to find usable subsequence on its pursuant biochemical properties and structural characteristics. Specifically, this work studies relationship between mentioned theoretically calculated property of subsequences and their efficiency for RNAi for any input set of experimental data. Furthermore, in this work software RNA Interference Analyzer is designed, implemented, tested and used.
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